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Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
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Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

Journal: Frontiers in Nutrition

Article Title: Hydrogen-rich water improves endurance by reducing skeletal muscle oxidative stress and inflammatory responses

doi: 10.3389/fnut.2026.1722091

Figure Lengend Snippet: Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

Article Snippet: Primary antibodies against myosin heavy chain isoforms were applied overnight at 4 °C (type I (BA-D5), IIa (SC-71), IIx (6H1), or IIb (BF-F3) (all from DSHB, Iowa City, IA, USA)).

Techniques: Purification, Muscles, Staining, Fluorescence